Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC

In this work, the characterization of several reversed-phase and HILIC chromatographic systems is presented by means Abraham's solvation parameter model, focusing on impact solute polarizability, dipolarity, hydrogen bonding, molecular volume retention. Although retention times in octadecylsilane columns are clearly dependent nature content organic modifier mobile phase, similar selectivities reported for eluents containing acetonitrile or methanol range between 40 80%. The most relevant analyte properties affecting bond acceptor capacity volume, former favoring partition into phase latter stationary phase. behavior greatly depends support (silica polymeric), bonded (zwitterionic, aminopropyl, dihydroxypropyl) solvent used eluent (acetonitrile methanol), but they have common that larger volumes allow more favorable solvent-rich evaluation ionized analytes should be performed with care, since may interact buffering species, leading to unexpected lower retentions.